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KMID : 0613820140240040393
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Journal of Life Science
2014 Volume.24 No. 4 p.393 ~ p.402
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Bacterial Community of Natural Dye Wastewater Treatment Facility
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Hwang Yeoung-Min
Kim Dae-Kuk
Lee Ji-Hee
Baik Keun-Sik
Park Chul
Seong Chi-Nam
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Abstract
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Culture-dependent and culture-independent denaturing gradient gel electrophoresis (DGGE) analyses were employed to investigate the bacterial community associated with a natural dye wastewater treatment facility. A total of 104 (influent water, 48 strains; aeration tank, 25; settling tank, 31) bacterial strains were isolated. Based on the 16S rRNA gene sequences comparison analysis, the isolates belonged to four phyla: Proteobacteria, Actinobacteria, Firmicutes, and Bacteriodetes. Seventeen DGGE bands representing dominant taxa in each sample were cloned and partially sequenced. The same four phyla were detected by DGGE fingerprinting. The most dominant taxon retrieved by both methods was the
member of the phylum Proteobacteria with Alphaproteobacteria as the predominant class. The bacterial community associated with the natural dye wastewater treatment facility is composed of parasites of animals and plants, decomposers of polysaccharides and dyes, and producers of extracellular polysaccharides.
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KEYWORD
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Bacterial community, denaturing gradient gel electrophoresis, natural dye wastewater
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